duoflow pathfinder 20 instrument (Bio-Rad)

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Bio-Rad duoflow pathfinder 20 instrument
Duoflow Pathfinder 20 Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/duoflow+pathfinder+20+instrument/BioLogic+DuoFlow+Pathfinder+20+System/pm36508984-113-7-11
Average 93 stars, based on 29 article reviews

duoflow pathfinder 20 instrument - by Bioz Stars, 2026-09

93/100 stars

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Article Title: Folded Monomers and Hexamers of the Ectodomain of the HIV gp41 Membrane Fusion Protein: Potential Roles in Fusion and Synergy Between the Fusion Peptide, Hairpin, and Membrane-Proximal External Region
Article Snippet: A DuoFlow Pathfinder 20 instrument (Bio-Rad) was used with a flow rate of 0.3 mL/min, A 280 detection, and Tricorn semipreparative columns (GE Technologies).

Injection:

Article Title: Efficient Fusion at Neutral pH by Human Immunodeficiency Virus gp41 Trimers containing the Fusion Peptide and Transmembrane Domains
Article Snippet: .. A ~100 μL aliquot was injected into a DuoFlow Pathfinder 20 instrument (Bio-Rad) equipped with a Tricorn Superdex 200 Increase 10/300 GL column (GE Technologies). ..

Chromatography:

Article Title: Full-Length Trimeric Influenza Virus Hemagglutinin II Membrane Fusion Protein and Shorter Constructs Lacking the Fusion Peptide or Transmembrane Domain: Hyperthermostability of the Full-Length Protein and the Soluble Ectodomain and Fusion Peptide Make Significant Contributions to Fusion of Membrane Vesicles
Article Snippet: .. The chromatography was done with a DuoFlow Pathfinder 20 instrument (Bio-Rad) with Tricorn Superdex 200 semi-preparative column (General Electric). ..

Size-exclusion Chromatography:

Article Title: A large HIV gp41 construct with trimer-of-hairpins structure exhibits V2E mutation-dominant attenuation of vesicle fusion and helicity very similar to V2E attenuation of HIV fusion and infection and supports: (1) hairpin stabilization of membrane apposition with larger distance for V2E; and (2) V2E dominance by an antiparallel β sheet with interleaved fusion peptide strands from two gp41 trimers.
Article Snippet: There is complete attenuation of fusion and infection mediated by HIV gp160 with gp41 subunit with V2E mutation, and also V2E dominance with WT/V2E mixtures.. V2E is at the N-terminus of the ~25-residue fusion peptide (Fp) which likely binds the target membrane.. In this study, large V2E attenuation and dominance were observed for vesicle fusion induced by FP_HM, a large gp41 ectodomain construct with Fp followed by hyperthermostable hairpin with Nand C-helices, and membrane-proximal external region (Mper).

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